Human eNOS/NOS3 ELISA Assay Kit
Human eNOS/NOS3 ELISA Assay Kit is for Research Use Only
Size: 1×96 wells
Sensitivity: <10pg/ml
Incubation period: < 280 Minutes
Standard Range: 78 pg/ml – 5,000 pg/ml
Sample Type: Cell culture supernates, Serum and Plasma
Sample Size: 100µL
Alternative Names: nitric oxide synthase 3
Assay Principle
The Eagle Bioscience Human NOS3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human NOS3 with a 96-well strip plate that is pre-coated with antibody specific for NOS3. The detection antibody is a biotinylated antibody specific for NOS3. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human NOS3 protein as standards.
To measure Human NOS3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human NOS3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human NOS3 in the sample.
