Human FLT3/FLK2 ELISA Kit

$450.00

The Eagle Biosciences Human FLT3/FLK2 ELISA Kit is intended for the quantitation of Human FLT3 concentrations in cell culture supernates, lysates, tissue, serum and plasma (heparin, EDTA). The Eagle Biosciences Human FLT3/FLK2 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FK231-K01 Categories: , ,

Human FLT3/FLK2 ELISA Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: <50pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Lysates, Tissue, Serum and Plasma (heparin, EDTA).
Sample Size: 100 µl

Additional Information

Assay Background


Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. It is mapped to 13q12.2. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Assay Principle


The Human FLT3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FLT3 with a 96-well strip plate that is pre-coated with antibody specific for FLT3. The detection antibody is a biotinylated antibody specific for FLT3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human FLT3 with immunogen: Expression system for standard: NSO, Immunogen sequence: N27-N541. The kit is analytically validated with ready to use reagents. To measure Human FLT3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FLT3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human FLT3 in the sample.

Typical Standard Curve


Manual

Product Manual