HCC-REAAD™ ERBB3 (HER3) ELISA (Hepatocellular Carcinoma -REcombinant Antigen-Antibody Detection) is intended for qualitative and semi-quantitative detection of human epidermal growth factor receptor 3 (ERBB3) proteins in human plasma/serum samples. The test utilizes sandwich ELISA using two layers of antibodies (i.e, capture and detection antibody) to determine a sample’s ERBB3 proteins levels in plasma / serum samples.

SKU: HE0100ER3 Categories: , ,


The HCC-REAAD ErbB3 (HER3) ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.3 RU
Standard Range: 0.3-0.7 RU
Incubation Time: 1.75 hours
Sample Type: Serum, plasma
Sample Size: 100 µl
Alternative Names: Hepatocellular Carcinoma -Recombinant Antigen-Antibody Detection, Receptor tyrosine-protein kinase erbB-3, HER3, Human Epidermal Growth Factor Receptor 3

Controls Included

Assay Principle

Human ERBB3 Capture Antibody is first coated onto the wells of microplates. Samples, standards and controls containing ERBB3 proteins are pipetted into these wells. During the first incubation, the protein antigen binds to the capture antibody and form antigen-antibody complexes. After washing, Human ERBB3 Detection Antibody is added to the wells and binds to the immobilised protein captured during the first incubation. After removal of excess detection antibody, a Horseradish Peroxidase (HRP)-conjugated Streptavidin is added and binds to the detection antibody. After a third incubation and washing to remove the excess HRP conjugate, a TMB substrate solution (tetramethylbenzidine) is added and is converted by the enzyme to a detectable form (color signal). The enzymatic reaction will then be stopped by the addition of 1N Sulphuric acid, which will turn the blue coloration to yellow. The microwells can be read on any suitable spectrophotometer or microwell ELISA plate reader. It is always recommended to read the wells at 450nm against a 620-630 nm reference filter to eliminate any possible causes of interference. The intensity of this colored product is directly proportional to the concentration of antigen present in the original specimen.

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Additional Information

Assay Background

Liver Cancer is the sixth most common cancer malignancy in the world with 83% of cases occurring in less developed countries [1]. Liver Cancer is the second leading cause of cancer death worldwide. The highest estimated mortality rates are in Eastern and South-Eastern Asia [1,3]. Hepatocellular Carcinoma (HCC) is the most common type of liver cancer, accounting for at least 70% of the total liver cancer cases [3]. HCC was once prevalent only in Southeast Asia and Africa due to dietary intake of aflatoxin contaminated food grains. However, in recent years, an increase of HCC incidences is discovered in Europe and United States [4]. Higher levels of ERBB3 proteins were detected in patients with HCC as compared to non-HCC patients [2].
HCC-REAAD™ ERBB3 (HER3) ELISA Assay Kit uses two layers of antibodies (i.e. capture and detection antibody) targeting at two different antigenic epitopes expressed by human ERBB3 protein capable of binding to the respective antibody to semi-quantify the level of human ERBB3 protein. The test uses an arbitrary unit of measurement known as REAAD™-units (RU).

Package Inserts

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