GC-REAAD™ ITIH3 ELISA Test (Gastric Carcinoma – Recombinant Antigen-Antibody Detection) is intended for qualitative and semi-quantitative detection of human Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) proteins in human plasma / serum. The test utilizes sandwich ELISA using two layers of antibodies (i.e. capture and detection antibody) to determine ITIH3 proteins levels in plasma / serum samples.


The GC-REAAD™ ITIH3 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.4 RU
Standard Range: 0.4-0.7 RU
Incubation Time: 2 hours
Sample Type: Serum, plasma
Sample Size: 100 µl
Alternative Names: Inter-alpha-trypsin inhibitor heavy chain 3, ITIH3 Protein, Human ITIH3, hITIH3, Gastric Carcinoma REAAD
Controls Included

Assay Principle

Human ITIH3 Capture Antibody is first coated onto the wells of microplates. Samples, standards and controls containing ITIH3 proteins are pipetted into these wells. During the first incubation, the protein antigen binds to the capture antibody and form antigen-antibody complexes. After washing, Human ITIH3 Detection Antibody is added to the wells and binds to the immobilised protein captured during the first incubation. After removal of excess detection antibody, a Horseradish Peroxidase (HRP) conjugate antibody is added and binds to the detection antibody. After a third incubation and washing to remove the excess HRP conjugate, a TMB substrate solution (tetramethylbenzidine) is added and is converted by the enzyme to a detectable form (color signal). The enzymatic reaction will then be stopped by the addition of 1N Sulphuric acid, which will turn the blue coloration to yellow. The microwells can be read on any suitable spectrophotometer or microwell ELISA plate reader. It is always recommended to read the wells at 450 nm against a 620-630 nm reference filter to eliminate any possible causes of interference. The intensity of this colored product is directly proportional to the concentration of antigen present in the original specimen.

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Additional Information

Assay Background

Gastric Carcinoma (GC) is the fifth most common cancer malignancy in the world with more than 70% of cases occur in developing countries, and half the world total occurs in Eastern Asia [3,5,6]. GC is the third leading cause of cancer death worldwide. The highest estimated mortality rates are in Eastern Asia. High mortality rates are also present in Central and Eastern Europe, and in Central and South America. The level of ITIH3 proteins has been found to be closely associated with Gastric Carcinoma. Higher levels of ITIH3 proteins were detected in plasma with gastric carcinoma (both early and late) as compared to plasma of non-carcinoma samples [1,2,4].
GC-REAAD™ sandwich ELISA uses two layers of antibodies (i.e. capture and detection antibody) targeting at two different antigenic epitopes expressed by human ITIH3 proteins capable of binding to the respective antibody to semi-quantify the level of human ITIH3 protein. The test uses an arbitrary unit of measurement known as REAAD™-units (RU). GC-REAAD™ is useful for the semi-quantification of human ITIH3 proteins in samples.

Package Inserts

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