Total Complement Functional Screen ELISA

$780.00

The Total Complement Functional Screen ELISA is an enzyme immunoassay for the qualitative determination of functional classical, MBL, and alternative complement pathways in human serum, the result shall not be used for clinical diagnosis or patient management. The Total Complement Functional Screen ELISA is for Research Use Only.

SKU: COMPL 300 Categories: , , Tags: ,

Total Complement Functional Screen ELISA

Total Complement Functional Screen ELISA Developed and Manufactured by Svar Life Science

Size: 1×96 wells
Sensitivity: See Package Insert
Dynamic Range: Cut-off
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 20 µL
For Research Use Only

Controls Included


Specimen Collection
Blood samples are to be collected using aseptic venipuncture technique and serum obtained using standard procedures. A minimum of 5 mL of whole blood is recommended. Allow blood to clot in serum tubes, for 60-65 minutes at room temperature (20-25° C). Centrifuge blood samples and transfer cell-free serum to a clean tube. Sera must be handled properly to prevent in vitro complement activation. Sera should be frozen at -70° C or lower in tightly sealed tubes for extended storage or for transport on dry ice. Samples should not be frozen and thawed more than once. Avoid using sera which are icteric, lipemic and hemolyzed. Heat-inactivated sera can not be used. Plasma can not be used.


Assay Principle

The Complement System Screen Kit combines principles of the hemolytic assay for complement activation with the use of labeled antibodies specific for neoantigen produced as a result of complement activation. The amount of neoantigen generated is proportional to the functional activity of complement pathways.

In the Total Complement Functional Screen ELISA Assay Kit the wells of the microtitre strips are coated with specific activators of the classical, or MBL, or the alternative pathway. Subject serum is diluted in diluent containing specific blocker to ensure that only the respective pathway is activated. During the incubation of the diluted subject serum in the wells, complement is activated by the specific coating.

The wells are then washed and C5b-9 is detected with a specific alkaline phosphatase-labeled antibody to the neoantigen expressed during MAC formation.

After a further washing step, detection of specific antibodies is obtained by incubation with alkaline phosphatase substrate solution. The amount of complement activation correlates with the color intensity and is measured in terms of absorbance (optical density (00)).

Related Products

Complement TCC ELISA Assay Kit
Complement Alternative Pathway ELISA Assay Kit
Complement C4d ELISA Assay Kit

Learn more about the Complement System at Eagle Biosciences Biomarker Spotlight page dedicated to the Complement System here:

EagleBio Spotlight: Complement System

Additional Information

Assay Background


The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the recently discovered MBL pathway.

Impaired complement activity causes humans to become susceptible to repetitive fulminant or severe infections and may contribute to the development of autoimmune disease. Inappropriate activation of complement contributes to chronic inflammation and tissue injury.

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Publications

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