Glutamine ELISA Assay Kit

$850.00

This Glutamine ELISA Assay is intended for the quantitative determination of L-Glutamine (Gln) in plasma samples. The Glutamine ELISA is for research use only and not to be used for diagnostic purposes.

Glutamine ELISA Assay Kit

Glutamine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol

Size: 1×96 wells
Sensitivity: 3µM
Standard Range: 21-3125µM
Incubation Time: Overnight
Sample Type: Serum and plasma (EDTA)
Sample Size: 10µL
Alternative Names: Gln
For Research Use Only

Controls Included


Specificity: No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine, and L-Glutamic acid.
Sample Collection and Storage
EDTA Plasma: Whole blood should be collected into centrifuge tubes containing EDTA as anti-coagulant and centrifuged according to manufacturer’s instructions at room temperature immediately after collection. Haemolytic and especially lipemic samples should not be used for the assay.
Storage: up to 48 hours at 2 – 8 °C, for longer period (up to 6 month) at -20 °C. Repeated freezing and thawing should be avoided.


Assay Principle

After extraction and derivatization L-Glutamine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.


Products Related to the Glutamine ELISA Assay Kit

L-Arginine ELISA Assay Kit
Quinolinic Acid ELISA Assay
Anticonjugated L-Serine Polyclonal Antibody

Additional Information

Assay Background


Glutamine (Gln) is an abundant non-essential amino acid that is involved in several physiological functions including the regulation of the immune system. However, in some disease conditions, Gln metabolism can be highly dysregulated. In cancer, Gln is highly consumed by tumor cells as to fulfill their bioenergetic and biosynthetic requirements for proliferation. Targeting Gln catabolism – mainly Glutaminase – is currently investigated as a therapeutic approach to circumvent high Gln consumption by cancer cells and maintain a sufficient level in the microenvironment for an effective anti-tumor immune response.

Typical Standard Curve


Documents

Product Manual