Glutamine ELISA Assay Kit
Glutamine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol
Size: 1×96 wells
Sensitivity: 3µM
Standard Range: 21-3125µM
Incubation Time: Overnight
Sample Type: Serum and plasma (EDTA), Cell Culture
Sample Size: 10µL
Alternative Names: Gln
For Research Use Only
Controls Included
Specificity: No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine, and L-Glutamic acid.
Sample Collection and Storage
EDTA Plasma: Whole blood should be collected into centrifuge tubes containing EDTA as anti-coagulant and centrifuged according to manufacturer’s instructions at room temperature immediately after collection. Haemolytic and especially lipemic samples should not be used for the assay.
Storage: up to 48 hours at 2 – 8 °C, for longer period (up to 6 month) at -20 °C. Repeated freezing and thawing should be avoided.
Assay Principle
After extraction and derivatization L-Glutamine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
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