Mouse GDF-15 ELISA Kit
The Mouse GDF-15 ELISA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 3.9pg/ml
Standard Range: 3.9pg/ml-250pg/ml
Incubation Time: 3 hours 35 minutes
Sample Type: Serum, plasma, and cell culture supernates
Sample Size: 100 µl
Alternative Names: Growth differentiation factor 15, TGF-PL, MIC-1, PDF, PLAB, PTGFB
Assay Principle
The Mouse GDF-15 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for mouse GDF-15. Standards and samples are pipetted into the wells and any mouse GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.
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