Complement Alternative Pathway ELISA
Complement Alternative Pathway ELISA Developed and Manufactured by Svar Life Science
Size: 1×96 wells
Sensitivity: See Package Insert
Dynamic Range: Cut-off
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Alternative Complement Pathway Immunoassay, Alternative Pathway Assay Kit
For Research Use Only
The Eagle Biosciences Complement Alternative Pathway ELISA Assay Kit combines principles of the hemolytic assay for complement activation with the use of labeled antibodies specific for neoantigen produced as a result of complement activation. The amount of neoantigen generated is proportional to the functional activity of the complement pathways.
In the Complement Alternative Pathway ELISA Assay kit, the wells of the microtitre strips are coated with specific activators of the alternative pathway. This in combination with sample dilution buffer composition and serum dilution level ensures that only the Alternative pathway is activated.
During the incubation of the diluted serum in the wells, complement is activated by the specific coating. The wells are then washed and the amount of C5b-9 complex formed on the plate surface is detected with a specific alkaline phosphatase labeled antibody to the C5b-9 neoantigen formed during MAC (Membrane Attack Complex) formation.
After a further washing step, detection of specific antibodies is obtained by incubation with alkaline phosphatase substrate solution. The amount of complement activation correlates with the color intensity and is measured in terms of absorbance (optical density (00).