Complement Alternative Pathway ELISA


The Eagle Biosciences Complement Alternative Pathway ELISA is an enzyme immunoassay for the qualitative and/or semi-quantitative determination of functional alternative complement pathway in human serum, the result shall not be used for clinical diagnosis or patient management. The Eagle Biosciences Human Complement Alternative Pathway ELISA Assay kit is for Research Use Only.

Complement Alternative Pathway ELISA

Complement Alternative Pathway ELISA Developed and Manufactured by Svar Life Science

Size: 1×96 wells
Sensitivity: See Package Insert
Dynamic Range: Cut-off
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Alternative Complement Pathway Immunoassay, Alternative Pathway Assay Kit
For Research Use Only

Controls Included

Assay Principle

The Eagle Biosciences Complement Alternative Pathway ELISA Assay Kit combines principles of the hemolytic assay for complement activation with the use of labeled antibodies specific for neoantigen produced as a result of complement activation. The amount of neoantigen generated is proportional to the functional activity of the complement pathways.

In the Complement Alternative Pathway ELISA Assay kit, the wells of the microtitre strips are coated with specific activators of the alternative pathway. This in combination with sample dilution buffer composition and serum dilution level ensures that only the Alternative pathway is activated.

During the incubation of the diluted serum in the wells, complement is activated by the specific coating. The wells are then washed and the amount of C5b-9 complex formed on the plate surface is detected with a specific alkaline phosphatase labeled antibody to the C5b-9 neoantigen formed during MAC (Membrane Attack Complex) formation.

After a further washing step, detection of specific antibodies is obtained by incubation with alkaline phosphatase substrate solution. The amount of complement activation correlates with the color intensity and is measured in terms of absorbance (optical density (00).

Related Products

Complement Classical Pathway ELISA Assay Kit
Complement C4d ELISA Assay Kit
Complement TCC ELISA Assay Kit

Additional Information

Assay Background

The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the lectin pathway.

Impaired complement activity causes humans to become susceptible to repetitive fulminant or severe infections and may contribute to the development of autoimmune disease. Inappropriate activation of complement contributes to chronic inflammation and tissue injury.

In vitro activation of the complement, sequence leads to the consumption of complement components which, in turn, leads to a decrease in their concentration. Thus, the determination of complement proteins or complement activity is used to indicate whether the complement system has been activated by an immunologic and/or pathogenic mechanism. Both functional and immunochemical complement measurements are used to evaluate subjects when a complement-activating disease is suspected or an inherited deficiency is possible. The level of complement activity evaluated by functional assays such as Eagle Biosciences Complement Alternative Pathway ELISA Assay Kit takes into account the rate of synthesis, degradation, and consumption of the components and provides a measure of the integrity of the pathways as opposed to immunochemical methods which specifically measure the concentration of various complement components.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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