Canine Albumin ELISA Assay

$330.00

The Canine Albumin ELISA Assay is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Dogs.

SKU: CAE49-K01 Categories: , ,

Canine Albumin ELISA Assay

The Canine Albumin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 2.463 ng/ml
Standard Range: 12.5-400 ng/ml
Incubation Time: 100 minutes
Sample Type: Canine Biological Fluids
Sample Size: 100 µl


Assay Principle for Canine Albumin ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of canine albumin in the sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. For urine samples, collect using standard techniques. The samples should be centrifuged to remove any possible debris. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.


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Additional Information

Assay Background


Albumin is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.

Typical Standard Curve


Manual

Product Manual