Estrone LIA Assay Kit
The Estrone LIA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 8.8 pg/mL
Dynamic Range: 15–2000 pg/mL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 50 μL
Controls Included
Assay Principle
The principle of the Estrone LIA Assay Kit chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of estrone in the sample. A set of calibrators are used to plot a standard curve from which the amount of estrone in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE for Estrone LIA
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
SPECIFICITY (CROSS-REACTIVITY)
The following compounds were tested for cross-reactivity with the estrone LIA kit with estrone cross-reacting at 100%:
Estrone 100%
Estrone-3-Sulfate 4.9%
17β-Estradiol 2.2%
Estrone-3-Glucuronide 1.2%
17ß-Estradiol-3-Glucuronide 0.14%
The following steroids were tested but cross-reacted at less than 0.1%: Androstenedione, Cholesterol, Corticosterone, Cortisol, Cortisone, DHEAS, Diethylstilbesterone, Estriol, 17ßEstradiol-3-Glucuronide, Estradiol-Sulfate, Progesterone, 17-OH Progesterone and Testosterone.
