The  DHEAS LIA Kit is for the direct quantitative determination of dehydroepiandrosterone sulfate (DHEAS) in human serum by a chemiluminescence immunoassay (LIA). The Eagle Biosciences DHEAS LIA kit is for research use only and not to be used in diagnostic procedures.

SKU: DHS31-L01 Categories: , ,


The DHEAS LIA Assay Kit is for research use only

Size: 1×96 wells
Sensitivity: 0.02 μg/mL
Dynamic Range: 0.02–10 μg/mL
Incubation Time: 60 minutes
Sample Type: Serum
Sample Size: 25 μL
Controls Included

Specimen Collection and Storage for DHEAS LIA Assay
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Human serum that may be used in the preparation of the standards and controls has been tested and found to be non- reactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.

Assay Principle

The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of DHEAS in the sample. A set of calibrators are used to plot a standard curve from which the amount of DHEAS in patient samples and controls can be directly read.

Related Products for Dehydroepiandrosterone sulfate LIA Assay

DHEA-S Saliva ELISA Assay Kit
DHEA-S Saliva Sensitive ELISA Assay Kit

Additional Information

Assay Background

DHEAS is one the most abundant circulating steroids. It is produced by the adrenal and gonads. As a result, the determination of the level of DHEAS in serum is important in the evaluation of the functional state of these glands. It is also a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. The principal production of testosterone in females is from the conversion of other related androgens especially DHEAS. An abnormal testosterone levels in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

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Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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