Estradiol Saliva ELISA Assay Kit


The Eagle Biosciences Estradiol Saliva ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Estradiol concentration in saliva. The Estradiol Saliva Assay Kit is intended for research use only and is not to be used for diagnostic procedures.

SKU: DCM022 Categories: , ,

Estradiol Saliva ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.5 pg/ml
Dynamic Range: 1 – 100 pg/ml
Incubation Time: 2.5 hour
Sample Type: Saliva
Sample Size: 100 µl

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Estradiol (17β-estradiol) is a sex hormone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning but also affects other organs including bone structure. During the reproductive years, most estradiol in women is produced by the ovaries, smaller amounts of estradiol are also produced by the adrenal cortex. In men, the testes produce estradiol. During pregnancy estrogen levels including estradiol rise steadily towards term. Estradiol increases due to placental production. In adult premenopausal women, ovarian estradiol production is stimulated by the interactions of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) throughout the menstrual cycle.  In adult women, estradiol levels are measured in the evaluation of fertility and menstrual irregularities, and to monitor ovarian follicular function during induction of ovulation. In the female, estradiol acts as a growth hormone for tissue of the reproductive organs. The development of secondary sexual characteristics in women is driven by estradiol. Estradiol is also involved in male fertility. Estradiol regulates bone maintenance. Women who past the menopause experience an accelerated loss of bone mass due to a relative estrogen deficiency. Estradiol affects the production of multiple proteins including lipoproteins, binding proteins, and proteins responsible for blood clotting. Estrogens have been found to have neuroprotective function. Estrogen is considered an oncogene as it supports certain cancers, notably breast cancer and cancer of the uterine lining. In addition, there are several benign gynecologic conditions that are dependent on estrogen such as endometriosis, leiomyomata uteri, and uterine bleeding.

Assay Principle

In the Estradiol Saliva Assay Kit, the estradiol (antigen) in the sample competes with the antigenic Estradiol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Estradiol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the Estradiol concentration of in the sample. Estradiol concentration in the sample is calculated through a calibration curve.


Product Manual