Estradiol Saliva ELISA Assay Kit


The Eagle Biosciences Estradiol Saliva ELISA Assay Kit is designed and validated for the quantitative measurement of 17ß-Estradiol in human saliva. The Estradiol Saliva ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: EST32-K01 Categories: , ,

Estradiol Saliva ELISA Assay Kit

The Estradiol Saliva ELISA Assay Kit is For Research Use Only

Size: 12 x 8 wells
Sensitivity: 0.5239 pg/ml
Standard Range: 1 – 6 pg/mL
Incubation Time: 2 hours 30 minutes
Sample Type: Saliva
Sample Size: 100 µL

Controls Included

Assay Principle for Estradiol Saliva ELISA

The Eagle Biosciences Estradiol Saliva ELISA Kit is based on the competition principal and microplate separation. An unknown amount of estradiol present in a saliva sample and a fixed amount of estradiol conjugated to horse radish peroxidase (E2-HRP) compete for binding sites with a rabbit monoclonal estradiol antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away. Enzyme substrate solution is then added, and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of E2-HRP detected is inversely proportional to the amount of estradiol in a sample.

Sample Collection and Processing

  • Avoid food consumption, drinking coffee or alcohol, smoking or chewing gum one (1) hour prior to sample collection.
  • Rinse mouth thoroughly with water 15 minutes prior to collection.
  • Collect whole saliva by unstimulated passive drool by allowing saliva to drip off the lower lip into a graduated plastic test tube (Eagle Biosciences recommends VWR Sample Mailing Tubes Cat #16465-260) or by allowing saliva to accumulate in the floor of the mouth and spitting it into the recommended collection device.
  • Time and date specimen. Refrigerate then freeze (-20°C or below) samples until day of assay. On day of assay, Thaw samples to facilitate precipitation of mucins. Centrifuge at 1500g for ten minutes. Bring samples to room temperature and assay.

Related Products to Ultrasensitive Cortisol Saliva ELISA

Estradiol ELISA Assay Kit
Rat Estradiol ELISA Assay Kit

Additional Information

Assay Background

17ß-Estradiol (E2) is a steroid hormone produced mainly by the Graafian follicle of the ovary female and in small amounts by the testes in male subjects. E2 is biologically the most active of naturally produced human estrogens. The majority of E2 (98%) is bound to sex hormone binding globulin (SHBH) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates in the free form or conjugated to sulfates and glucuronides. In non-pregnant women there is a cyclic variation in the concentration of E2, the highest values being measured usually the day before ovulation. Positive feedback influence of this peak value is considered essential for occurrence of the midcycle luteinizing hormone (hLH) peak and consequently, for ovulation. During pregnancy the E2 concentration increases considerably and remains high throughout pregnancy.

The assay of E2 is a valuable tool for assessing the etiology of amenorrhea and/or infertility in female subjects. It is also a useful aid in monitoring ovulation induction treatment with clomiphene citrate, LH-RH (LH-releasing hormone) or exogenous gonadotropins. In male subjects, serum E2 measurements are used for investigating feminizing syndromes.

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