Estradiol Saliva ELISA Assay Kit
The Estradiol Saliva ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.5239 pg/ml
Standard Range: 1 – 6 pg/mL
Incubation Time: 2 hours 30 minutes
Sample Type: Saliva
Sample Size: 100 µL
Assay Principle for Estradiol Saliva ELISA
The Eagle Biosciences Estradiol Saliva ELISA Kit is based on the competition principal and microplate separation. An unknown amount of estradiol present in a saliva sample and a fixed amount of estradiol conjugated to horse radish peroxidase (E2-HRP) compete for binding sites with a rabbit monoclonal estradiol antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away. Enzyme substrate solution is then added, and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of E2-HRP detected is inversely proportional to the amount of estradiol in a sample.
Sample Collection and Processing
- Avoid food consumption, drinking coffee or alcohol, smoking or chewing gum one (1) hour prior to sample collection.
- Rinse mouth thoroughly with water 15 minutes prior to collection.
- Collect whole saliva by unstimulated passive drool by allowing saliva to drip off the lower lip into a graduated plastic test tube (Eagle Biosciences recommends VWR Sample Mailing Tubes Cat #16465-260) or by allowing saliva to accumulate in the floor of the mouth and spitting it into the recommended collection device.
- Time and date specimen. Refrigerate then freeze (-20°C or below) samples until day of assay. On day of assay, Thaw samples to facilitate precipitation of mucins. Centrifuge at 1500g for ten minutes. Bring samples to room temperature and assay.