Anti-tTG sIgA/IgA ELISA Assay Kit

$520.00

The Eagle Biosciences Anti-tTG sIgA/IgA ELISA Assay Kit is intended for the quantitative determination of anti-human tissue transglutaminase sIgA/IgA in stool. The Anti-tTG sIgA/IgA ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

SKU: IC6400 Categories: , ,

Anti-tTG sIgA/IgA ELISA Assay Kit

The Anti-tTG sIgA/IgA ELISA Assay Kit is For Research Use Only

Size: 12 x 8 wells
Sensitivity: 2.1 mU/g
Incubation Time: 2 hours 15 minutes
Sample Type: Stool
Sample Size: 50 µl
Controls Included

Product Developed and Manufactured in Germany
Product Support in the United States


Assay Background

Celiac disease is a chronic illness of the small intestinal mucous membrane. The reason is an intolerance against gluten, which is found in many cereals. This food intolerance against cereal proteins leads to a production of auto-antibodies against tissue transglutaminase, which is the major antigen for endomysium antibodies.

The intake of gluten-containing food leads to an inflammation of the small intestinal mucous membrane, which results in a reduced absorption of nutrients. The symptoms of the disease are reduction of weight, diarrhea, vomitus, anorexia and tiredness. The growth of children is impaired. The characteristic of the symptoms might be different. The only therapeutic treatment is a gluten-free diet. A non-treated celiac disease increases the risk of non-Hodgkin-lymphoma and colon cancer. Celiac disease is associated with type 1 diabetes mellitus in five to ten percent of the patients. Women are more often affected than men. The outcome of the disease is pronounced during infancy and in ages between 30 and 40 years old.

Applications:

  • Food Intolerance
  • Monitoring of an elimination diet

The Eagle Biosciences Anti-human tissue transglutaminase sIgA / IgA kit allows an easy, rapid and precise quantitative determination of the antibodies in biological samples. The kit includes all reagents ready to use for preparation of the samples.


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Additional Information

Assay Principle


The anti-tissue transglutaminase-ELISA test determines anti-tissue transglutaminase sIgA / IgA antibodies according to the “sandwich”-principle. Anti-tissue transglutaminase antibodies in sample, standard and controls bind to tissue transglutaminase, which is coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are measured at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The anti-tissue transglutaminase concentration can be calculated from the standard curve.

Standard Curve

Anti-tTG sIgA/IgA

Documents

Product Manual