E. coli O157 ELISA Assay Kit
E. coli O157 ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Stool
Sample Size: 250 mg
Alternative Names: Enterohemorrhagic Escherichia coli O157:H7, E. Coli O157:H7
For Research Use Only
Specificity: The E. coli O157 ELISA Assay Kit does not cross react to the following: Toxin A, Toxin B, Helicobacter pylori, glutamate dehydrogenase 1 (GDH), Cryptosporidium parvum, Giardia lamblia, rotavirus and adenovirus.
Precision: The precision of this assay is validated by measuring two samples both in a single assay of 16-replicate determinations (intra-assay)
Sample 1: Mean 1.687, Std Dev 0.049, %CV 2.9%
Sample 2: Mean 1.975, Std Dev 0.058, %CV 2.9%
Most E. coli strains harmlessly colonize the gastrointestinal tract of humans and animals as a normal flora. However, there are some strains that have evolved into pathogenic E. coli by acquiring virulence factors through plasmids, transposons, bacteriophages, and/or pathogenicity islands.
Enterohemorrhagic Escherichia coli O157:H7, also known as EHEC, is a major foodborne pathogen causing severe disease in humans worldwide. Healthy cattle are a reservoir of E. coli O157:H7, and bovine food products and fresh produce contaminated with bovine waste are the most common sources for disease outbreaks in the United States. E. coli O157:H7 also survives well in the environment.
E. Coli O157:H7 is the most predominant strain of Shiga Toxin producing E. Coli in the United States, Japan, and the United Kingdom. The Centers for Disease Control and Prevention (CDC) has estimated that E. coli O157:H7 infections cause 73,000 illnesses, 2,200 hospitalizations, and 60 deaths annually in the United States. Healthy adults usually recover from infection with E. coli O157:H7 within a week, but young children and older adults have a greater risk of developing a life-threatening form of kidney failure called hemolytic uremic syndrome.