Anti-IgE Antibody ELISA Kit
Anti-IgE Antibody ELISA Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.4808 ng/mL
Dynamic Range: 12 – 450 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 200 µL
Alternative Names: Immunoglobulin E
For Research Use Only
This Anti-IgE Antibody ELISA Kit is designed, developed and produced for the quantitative measurement of human anti-hIgE autoantibodies in serum and plasma samples. The assay utilizes the two-site “bridge” technique with two selected human antibodies.
Assay standards, controls and patient samples are directly added to wells of a microplate that is coated with purified human IgE. After the first incubation period, anti-IgE antibodies bind to the human IgE on the wall of microtiter well and unbound proteins in each microtiter well are washed away. A highly purified biotin-labeled human IgE is then added to each microtiter well. After the second incubation period, and a “bridge” of “well coated human IgE = Anti-hIgE antibody = biotin-labeled human IgE” is formed. The unbound protein is removed in the subsequent washing step. HRP-labeled streptavidin is added to the plate wells. For the detection of this immunocomplex, the wells are then incubated with a substrate solution in a timed reaction and subsequently measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the IgE on the wall of the microtiter well is directly proportional to the amount of anti-IgE antibody in the sample. A standard curve is generated by plotting the absorbance versus the respective anti-IgE concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of IgE antibody in test samples is determined directly from this standard curve.