Corticosterone EIA Kit
The Corticosterone EIA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 18.6 pg/ml
Dynamic Range: 78.125 – 10,000 pg/ml
Incubation Time: 1.5 hours
Sample Type: Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL
Product manufactured in Canada by StressMarq.
Assay Principle
This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
