Celiac EmA IgA ELISA


The Celiac EmA (Anti-Endomysium Antibody) IgA ELISA assay kit is used for the quantitative determination of IgA autoantibodies to sample endomysial autoantigens (EmA).   The Eagle Biosciences Celiac EmA (Anti-Endomysium Antibody) IgA ELISA assay kit is for research use only and not to be used in diagnostic procedures.
This product was previously known as CEH31-K01.

Celiac EmA IgA ELISA

Celiac EmA IgA ELISA Developed and Manufactured by Medipan

Size: 1×96 wells
Sensitivity: 1 U/mL
Dynamic Range: 1 – 300 U/mL
Incubation Time: 1 hour, 45 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Celiac Anti-Endomysium Antibody IgA ELISA, Human Celiac EMA IgA ELISA,
For Research Use Only

Assay Principle

Celiac EmA (Anti-Endomysium Antibody) ELISA assay kit is an enzyme immunoassay for the quantitative determination of IgA autoantibodies to the supposed major autoantigens in Dermatitis herpetiformis and celiac disease in sample serum.  Autoantibodies of the diluted patient samples and calibrators react with the immobilized human autoantigens on the solid phase of the microtiter plate. Celiac EmA (Anti-Endomysium Antibody) ELISA assay kit guarantees the specific binding of autoantibodies usually found by immunofluorescence on endomysial antigens. Following an incubation period of 60 min at room temperature (18…25°C) unbound serum components are removed by a wash step.

Bound autoantibodies react specifically with anti-human-IgA-antibodies conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at room temperature. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.

HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl­benzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min at room temperature turning the solution from blue to yellow.  The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound.  The standard curve is established by plotting the concentrations of the antibodies of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.

Related Products

Celiac EmA (Anti-Endomysium Antibody) IgG ELISA Assay Kit
Gliadin IgA ELISA Kit
Anti-tTG IgA ELISA Kit

Additional Information

Typical Standard Curve

Celiac EmA IgA ELISA

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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