Celiac EmA IgG ELISA
Celiac EmA IgG ELISA Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 1.0 U/ml
Dynamic Range: 1 – 300 U/ml
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Celiac Anti-Endomysium Antibody IgG ELISA, Human Celiac EmA IgG ELISA, Human Endomysial Autoantigens IgG ELISA
For Research Use Only
Celiac EmA IgG ELISA assay kit is an enzyme immunoassay for the quantitative determination of IgG autoantibodies to the supposed major autoantigens in Dermatitis herpetiformis and celiac disease in sample serum.
Autoantibodies of the diluted samples and standards react with the immobilized human autoantigens on the solid phase of the microtiter plate. Celiac EmA (Anti-Endomysium Antibody) IgG ELISA assay kit guarantees the specific binding of autoantibodies usually found by immunofluorescence on endomysial antigens. Following an incubation period of 60 min at room temperature (18-25°C), unbound serum components are removed by a wash step.
The bound autoantibodies react specifically with anti-human-IgG-antibodies conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at room temperature. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min at room temperature turning the solution from blue to yellow.
The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the concentrations of the antibodies of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Evaluating the test by a semi-quantitative method is also possible.
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