Celiac EmA IgA ELISA
Celiac EmA IgA ELISA Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 1 U/mL
Dynamic Range: 1 – 300 U/mL
Incubation Time: 1 hour, 45 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Celiac Anti-Endomysium Antibody IgA ELISA, Human Celiac EMA IgA ELISA,
For Research Use Only
Assay Principle
Celiac EmA (Anti-Endomysium Antibody) ELISA assay kit is an enzyme immunoassay for the quantitative determination of IgA autoantibodies to the supposed major autoantigens in Dermatitis herpetiformis and celiac disease in sample serum. Autoantibodies of the diluted patient samples and calibrators react with the immobilized human autoantigens on the solid phase of the microtiter plate. Celiac EmA (Anti-Endomysium Antibody) ELISA assay kit guarantees the specific binding of autoantibodies usually found by immunofluorescence on endomysial antigens. Following an incubation period of 60 min at room temperature (18…25°C) unbound serum components are removed by a wash step.
Bound autoantibodies react specifically with anti-human-IgA-antibodies conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at room temperature. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the concentrations of the antibodies of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.
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