Anti-Pseudomonas aeruginosa IgG ELISA

$2,050.00

The Eagle Biosciences anti-Pseudomonas aeruginosa IgG ELISA Assay Kit detects qualitatively and semi-quantitativley IgG-Antibodies in human serum against the extracellular proteins: Alkaline Protease (AP), Elastase (Ela) and Exotoxin A (Exo) of Pseudomonas aeruginosa. This kit is For Research Use Only and not for diagnostic procedures.

Anti-Pseudomonas aeruginosa IgG ELISA

The Anti-Pseudomonas aeruginosa IgG ELISA is For Research Use Only

Size: 3 x 96 wells
Sensitivity: 0.02 ng/ml
Incubation Time: 4.75 hours
Sample Type: Serum, Plasma
Sample Size: 10 ul

Product manufactured by Mediagnost


Assay Principle

The Eagle Biosciences anti-Pseudomonas aeruginosa IgG anti-Pseudomonas aeruginosa IgG EIA, E15, is a sandwich enzyme immunoassay. Serum or plasma samples are diluted and added to the wells of a microtiter plate, which have been previously coated with the Pseudomonas aeruginosa antigens alkaline protease, elastase or exotoxin A. Specific antibodies in the sample bind to the antigens present during an incubation of 2h at 37°C. After washing, the conjugate (anti-human IgG peroxidase-labelled immunoglobulin) is added and incubated again (for 2 h at 37°C). After a final washing step, substrate is added and further incubated for 30 min at room temperature. The reaction is terminated on addition of stop solution accompanied by a change from blue to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample.


SPECIMEN COLLECTION, PREPARATION AND STORAGE
Serum samples are suitable. A special external sample preparation prior to assay is not required. Slight hemolysis of the samples doesn’t disturb the determination. Samples should be handled as recommended in general: as fast as possible and chilled as soon as possible. In case there will be a longer period between the sample withdrawal and determination, store the undiluted samples frozen -20°C or below in tightly closable plastic tubes. Avoid on principal repeated freeze-thaw cycles of serum/plasma (if required, please subaliquote).


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Additional Information

Assay Background


Pseudomonas aeruginosa, a Gram-negative bacterium ubiquitously distributed in the moist environment, causes about 10% of all nosocomial infections. This opportunistic pathogen leads to acute and chronic types of infection within the various organs.  P. aeruginosa infection provokes a rapid production of antibodies to a large number of P. aeruginosa antigens. Eagle Biosciences’s sensitive antibody detection system detects a P.aeruginosa infection very early at the onset.  Due to the use of three P.aeruginosa antigens, which are highly immunogenic and present in different parts from nearly all P.aeruginosa strains.  Depending on the Pseudomonas aeruginosa species and the immune reaction, antibodies can be detected against a single, two or even all three antigens simultaneously. A sample is regarded as sero-positive when antibodies against one or more of the antigens can be detected.

Anti-Pseudomonas aeruginosa IgG ELISA

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