Anti-Pseudomonas aeruginosa IgG ELISA
The Anti-Pseudomonas aeruginosa IgG ELISA is For Research Use Only
Size: 3 x 96 wells
Sensitivity: 0.02 ng/ml
Incubation Time: 4.75 hours
Sample Type: Serum, Plasma
Sample Size: 10 ul
Product manufactured by Mediagnost
The Eagle Biosciences anti-Pseudomonas aeruginosa IgG anti-Pseudomonas aeruginosa IgG EIA, E15, is a sandwich enzyme immunoassay. Serum or plasma samples are diluted and added to the wells of a microtiter plate, which have been previously coated with the Pseudomonas aeruginosa antigens alkaline protease, elastase or exotoxin A. Specific antibodies in the sample bind to the antigens present during an incubation of 2h at 37°C. After washing, the conjugate (anti-human IgG peroxidase-labelled immunoglobulin) is added and incubated again (for 2 h at 37°C). After a final washing step, substrate is added and further incubated for 30 min at room temperature. The reaction is terminated on addition of stop solution accompanied by a change from blue to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample.
SPECIMEN COLLECTION, PREPARATION AND STORAGE
Serum samples are suitable. A special external sample preparation prior to assay is not required. Slight hemolysis of the samples doesn’t disturb the determination. Samples should be handled as recommended in general: as fast as possible and chilled as soon as possible. In case there will be a longer period between the sample withdrawal and determination, store the undiluted samples frozen -20°C or below in tightly closable plastic tubes. Avoid on principal repeated freeze-thaw cycles of serum/plasma (if required, please subaliquote).