Anti-M2 ELISA Assay Kit
Anti-M2 ELISA Assay Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 3 U/ml
Dynamic Range: 3 – 300 U/ml
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Human Anti-M2 ELISA, Mitochondrial Antibody ELISA, Human Mitochondrial Antibody ELISA
For Research Use Only
Selected positive serum samples have been tested by this assay and found to dilute linearly. However, due to the heterogeneous nature of human autoantibodies there might be sera that do not follow this rule.
No cross reactivity to other autoantigens have been found.
The group of primary autoimmune liver disease (PAL) comprises autoimmune hepatitis (AIH), PBC and primary sclerosing cholangitis (PSC).
The clinical picture of PAL is in most cases not different from other chronic liver diseases. About 15% of all cases with chronic liver diseases show an autoimmune pathogenesis. Therefore, after exclusion of infectious etiology especially by viruses, the determination of different autoantibodies is recommended.
PBC is a chronic inflammatory disorder of the small and medium bile ducts and serologically characterized by the occurrence of circulating M2 autoantibodies. M2 autoantibodies belong to the group of mitochondrial antibodies (AMA) and recognize three related proteins of the alpha-keto acid dehydrogenase complex. This complex is located at the inner mitochondrial membrane. The major epitopes of this protein complex is found on the E2 subunit and the protein X of the pyruvate dehydrogenase complex (PDC). Furthermore, M2 autoantibodies bind the E1alpha and E1beta subunits of the same complex and the E2 subunit of several other multi-enzyme complexes, such as the 2-oxo-glutarate dehydrogenase complex (OGDC) and the branched chain 2-oxo acid dehydrogenase complex (BCOADC).
M2 autoantibodies have been found in up to 96% of patients suffering from PBC thus representing a powerful serological tool for the diagnosis of PBC.