High Sensitive Anti-Tg ELISA
High Sensitive Anti-Tg ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 1 U/mL
Dynamic Range: 20 – 1280 U/ml
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Anti-Tg IgG, Thyroglobulin IgG, Immunoglobulin G
For Research Use Only
The anti-Tg ELISA is designed, developed and produced for the quantitative measurement of human anti-Tg IgG autoantibody level in test sample. Assay calibrators, controls and pre-diluted human serum samples containing anti-Tg IgG autoantibody are added to microtiter wells of microplate that was coated with high affinity streptavidin on its wall. The autoantibody reaction will not start until the addition of a biotinylated human Tg antigen. After the first incubation period, the unbound protein matrix is removed in the subsequent washing step. A horseradish peroxidase-conjugated rabbit anti-human IgG subclass specific antibody (tracer antibody) is added to each well.
After an incubation period an immunocomplex of “solid-phase bound biotin-Tg – human anti-Tg IgG – HRP-conjugated tracer antibody” is formed if there is human anti-Tg IgG autoantibody present in the test sample. The unbound tracer antibody is removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the human IgG on the wall of the microtiter well is directly proportional to the amount of human anti-Tg IgG autoantibody level in the sample. Plotting the absorbance versus the respective human anti-Tg IgG autoantibody concentration for each calibrator on point-to-point or 4-parameter fit generates a calibrator curve. The concentration of human anti-Tg IgG autoantibody in test samples is determined directly from this calibrator curve.