TRAb clone ELISA Kit

$525.00

The TRAb clone ELISA Assay Kit is used for the quantitative determination of Thyrotropin (TSH) receptor autoantibodies in human serum. This Eagle Biosciences TRAb clone ELISA is for research use only and not for use in diagnostic procedures.

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TRAb clone ELISA Kit

TRAb clone ELISA Kit Developed and Manufactured by Medipan

Size: 1×96 wells
Sensitivity: 1.08 IU/l
Dynamic Range: 0.1 – 40 IU/l (WHO 08/204)
Incubation Time: 3 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: TSH Receptor Auto Antibody ELISA, Human TRAb clone ELISA
For Research Use Only

Controls Included


Assay Principle

The TRAb clone ELISA Kit is a competitive enzyme immunoassay with a step by step incubation. During the first incubation the TSH receptor antibodies of the patient samples and calibrators bind to the immobilized human recombinant receptor on the solid phase of the microtiter plate. Following an incubation period of 120 min, unbound antibodies are separated from the solid-phase immune complexes by a washing step. In a second incubation step of 20 min a biotinylated tracer antibody binds to the free epitopes of the receptor. The absence of serum autoantibodies against TSH receptor results in a complete saturation of the provided receptor by the biotinylated antibody. The presence of serum autoantibodies (TRAb) decreases the amount of biotinylated antibody bound. Unbound biotin complexes are removed by a washing step.

The complexed biotin reacts specifically with streptavidin horseradish peroxidase (HRP) conjugate. After the incubation period of 20 min, excessive conjugate is separated from the solid-phase immune complexes by the following washing step. Horseradish peroxidase converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 20 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is indirectly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the concentrations of the antibodies of the standards (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.


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Additional Information

Assay Background


TRAb clone (TSH Receptor Autoantibodies) ELISA Assay Kit is used for the quantitative determination of Thyrotropin (TSH) receptor autoantibodies in human serum.  Whereas thyrotoxicosis is caused by thyroid autonomy, hyperthyroidism of Graves’ disease is due to TSH receptor autoantibodies (TRAb). These autoantibodies mimic TSH effects on the thyroid cell and thus increase blood levels of T4 and T3.   Consequently, the measurement of TRAb is valuable for the differential diagnosis of hyperthyroidism as well as for the follow-up of Graves’ disease, both during and after its treatment by antithyroid drugs, 131-radioiodine or surgery. Possible additional diagnostic relevance has to be established in the future.

Typical Standard Curve


TRAb clone ELISA Kit Standard Curve

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