Anti-LKM1 ELISA Assay Kit

$525.00

The Anti-LKM1 ELISA Assay Kit is used for the quantitative deter­mination of IgG antibodies to cytochrome P450 IID6 in human serum or plasma.  The Eagle Biosciences Anti-LKM1 (Liver-Kidney Microsomes) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: LK131-K01 Categories: , , Tags: ,

Anti-LKM1 ELISA Assay Kit

Anti-LKM1 ELISA Assay Kit Developed and Manufactured by Medipan

Size: 1×96 wells
Sensitivity: 3 U/ml
Dynamic Range: 3 – 300 U/ml
Incubation Time: 90 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Liver Kidney Microsome ELISA, Liver-Kidney Microsome Antibody ELISA, Human Anti-LKM1 ELISA
For Research Use Only


Specificity for Liver-Kidney Microsome Antibody ELISA
No cross reactivity to other auto antigens have been found.

Assay Principle

Anti-LKM1 ELISA Assay Kit (Liver-Kidney Microsomes) is an enzyme immunoassay for the quantitative  determination of IgG antibodies to cytochrome P450 IID6.   The antibodies of the standards, controls, and diluted samples react with human recombinant cytochrome P450 IID6 immobilized on the solid phase of microtiter plates. The use of highly purified sample recombinant cytochrome P450 IID6 guarantees the specific binding of LKM-1 antibodies of the specimen under investigation. Following an incubation period of 30 min at room temperature (RT), unbound serum components are removed by a wash step.

The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.  HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl­benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (HCl) into the wells after 30 min at RT turning the solution from blue to yellow.  The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.


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Additional Information

Assay Background


The Anti-LKM1 (Liver-Kidney Microsomes) ELISA Assay Kit is used for the quantitative deter­mination of IgG antibodies to cytochrome P450 IID6 in human serum or plasma.  The group of primary autoimmune liver disease (PAL) comprises AIH, primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC).  The clinical picture of PAL is in most cases not different from other chronic liver diseases. About 15% of all cases with chronic liver diseases show an autoimmune pathogenesis. Therefore, after exclusion of infectious etiology especially by viruses, the determination of different autoantibodies is recommended.

Typical Standard Curve


Anti LKM1 ELISA Standard Curve

Documents


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