SLPI Protein ELISA Assay

$870.00

The Eagle Biosciences Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA Kit is intended for the quantitative determination of the Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) concentration in human serum.  The Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA assay kit is for research use only and not to be used in diagnostic procedures.

SKU: SLP31-K01 Categories: , ,

SLPI Protein ELISA Assay

The SLPI Protein ELISA Assay is For Research Use Only
Product made in the USA
Size: 1×96 wells
Sensitivity: 1.0 ng/ml
Dynamic Range: 5 – 80 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum
Sample Size: 50 µL
Alternative Names: Secretory Leukocyte Peptidase Inhibitor
Controls Included


Assay Background

The Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA assay kit is a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. The SLPI (ALP) protein is also thought to have broad-spectrum antibiotic activity.
Secretory Leukocyte Peptidase Inhibitor Protein has been known by many names, including SLPI, ALP Protein, Antileukoproteinase protein, ALK1 protein, ALP protein, BLPI protein, HUSI protein, HUSI-I protein, MPI protein, WAP4 protein, WFDC4 protein, secretory leukocyte peptidase inhibitor protein, ALK 1 protein, Antileukoproteinase 1 protein, Antileukoproteinase1 protein, HUSI 1 protein, HUSI I protein, Protease inhibitor WAP4 protein, Seminal proteinase inhibitor protein, WAP four disulfide core domain protein 4 protein, WAP 4 protein, or WFDC 4.


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Additional Information

Assay Procedure


  1. Dispense 50µl of SLPI standards, calibrators, and specimens into appropriate wells.
  2. Dispense 50µl of anti-SLPI-HRP Reporter into each well and incubate at room temperature for 2 hours with gentle agitation.
  3. Remove samples by emptying the plate contents into a waste container
  4. Remove liquid from all wells. Wash wells three times with 300 µl of 1X was buffer. Blot on absorbance paper or paper towels after each wash.
  5. Strike the microtiter plate sharply onto absorbance paper or paper towels to remove all residual liquid droplets.
  6. Dispense 100µl of TMB Reagent into each well and incubate at room temperature in the dark for 30 minutes.
  7. Stop the reaction by adding 100µl of Stop Solution into each well.
  8. Gently mix for 30 seconds. It is important to make sure that all the blue color changes to yellow color completely.
  9. Read the optical density at 450nm with a microtiter plate reader within 15 minutes.

Manual

Product Manual