Adrenaline, Noradrenaline & Dopamine Assay kit
Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika EA603/288
Size: 3 x 96 wells (1 plate for Adrenaline, 1 plate for Noradrenaline, 1 plate for Dopamine)
Sensitivity:
Urine: Adrenaline: 0.08 ng/ml, Noradrenaline: 0.67 ng/ml, Dopamine 0.43 ng/ml
Plasma: Adrenaline: 5 pg/ml, Noradrenaline: 45 pg/ml, Dopamine 29 pg/ml
Dynamic Range: Adrenaline: 0.5 – 250 ng/ml, Noradrenaline: 1.5 – 500 ng/ml, Dopamine: 1.5 – 2560 ng/ml
Incubation Time: Overnight
Sample Type: Urine, EDTA Plasma
Sample Size: Urine: 20 µl, Plasma: 300 µl
Alternative Names: Epinephrine, Norepinephrine
For Research Use Only
Controls Included
Normal Values
Urine: Adrenaline < 20 µg/day, Noradrenaline < 90 µg/day, Dopamine < 600 µg/day
Plasma: Adrenaline < 100 pg/ml, Noradrenaline < 600 pg/ml, Dopamine < 100 pg/ml
It is suggested that each laboratory establish its own normal ranges
Assay Background
Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.
An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglioneuroma). An increased or decreased concentration of the catecholamines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The measurement of dopamine and its derivatives is of special diagnostic value with children who are suspected to have a neuroblastoma. The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine. Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormetanephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.
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