Ultra-Sensitive Streptavidin Coated Clear 96 Well Microplate

$215.00$655.00

The Ultra-Sensitive Streptavidin Coated Clear 96 Well Microplate is a pre-blocked, clear 12 x 8-well, strip microplate that can be used for specific capture of biotin and biotinylated molecules.  These microplates are intended For Research Use Only and are not to be used for Diagnostic or Therapeutic Procedures.

Clear

Ultra-Sensitive Streptavidin Coated Clear 96 Well Microplate

The Ultra-Sensitive Streptavidin Coated Clear 96 Well Microplate is For Research Use Only

The Ultra-Sensitive Streptavidin Coated 96 Well Clear Microplate is a solid support for the binding of biotinylated molecules in a variety of assays for the measurement of drugs, peptides, proteins, DNA/RNA (nucleic acids), and a host of other molecules. These microplates are not intended for capture and release of biotinylated molecules as the streptavidin is passively, not covalently, bound to the microplates.  Any process used to release the biotinylated antibody from the streptavidin will, in all likelihood, release the streptavidin from the microplate.

Product Developed and Manufactured in the USA


Available Sizes:

  • 5 Plates (STP00-K05)
  • 20 Plates (STP00-K20)
  • Bulk Options are available.  Contact Eagle Biosciences for details.

Assay Background

Streptavidin (MW ~55,000 Daltons) is a tetrameric protein isolated from Streptomyces avidinii, which specifically binds biotin (244 Daltons).  The binding between streptavidin and biotin is one of the strongest non-covalent bindings known at (Kd) of ~10-14 mol/L (biotin-streptavidin complex).Unlike avidin, streptavidin in non-glycosylated, and is mildly acidic (pI~5.5), whereas avidin is basic (pI~10.5).  Because streptavidin lacks glycosylated moieties and has a lower pI than avidin, streptavidin has considerably less non-specific binding which results in less background signal in many tests.  These characteristics make streptavidin an ideal choice for a variety of applications including studies with cells, nucleic acids, drugs, small/large molecules, peptides, and proteins.


Related Products

Ultra-Sensitive Streptavidin Coated 96 Well Microplate (White)

Additional Information

Product Characteristics


  • Limit of Detection*:  2 ng/mL (biotinylated Mouse IgG)
  • Range*:  2-2,500 ng/mL (biotinylated Mouse IgG)
  • Precision:  <5% between individual wells.<8% between plates
  • Specificity:  Biotin or Biotinylated molecules

*As determined by colorimetric assay using biotinylated Mouse IgG and goat anti-mouse IgG-HRP for detection.

Manual

Product Manual