5-HIAA ELISA Assay Kit

$450.00

The 5-HIAA ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of 5-Hydroxy-3-Indole Acetic Acid (5-HIAA) in urine.

SKU: BA E-1900 Categories: , ,

5-HIAA ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.17 mg/l
Standard Range: 0.5 – 50 mg/l
Incubation Time: 2.5 hours
Sample Type: Urine
Sample Size: 50 µl

Controls Included

Additional Information

Assay Background


5-HIAA (5-hydroxyindoleacetic acid) is the major urinary metabolite of serotonin, an ubiquitous bioactive amine. Serotonin, and consequently 5-HIAA, is produced in excess by most carcinoid tumors, especially those associated with the carcinoid syndrome. The syndrome includes flushing and diarrhea, and, less frequently, heart failure and bronchoconstriction. Quantitation of urinary 5-HIAA is therefore intended to test for carcinoid.

Assay Principle


First, 5-HIAA is derivatized by methylation. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The methylated analyte in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system has reached equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Manual

Product Manual