Tryptophan ELISA Assay Kit
For Research Use Only
Size: 1×96 wells
Sensitivity: 1.2 µg/ml
Standard Range: 2.5 – 250 µg/ml
Incubation Time: overnight, 2 x 30 min
Sample Type: Urine, plasma, serum
Sample Size: 20 µl
Controls Included
Assay Principle
After extraction and derivatization Tryptophan is quantitatively determined by ELISA.
The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
