17α-Hydroxyprogesterone ELISA Assay Kit
The 17α-Hydroxyprogesterone ELISA Assay Kit is for Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.058 ng/mL
Dynamic Range: 0.05–30 ng/mL
Incubation Time: 1 hour 20 minutes
Sample Type: Serum
Sample Size: 20 µL
Assay Principe for 17α-Hydroxyprogesterone ELISA
The Eagle Biosciences 17α-Hydroxyprogesterone (17α-OHP) ELISA is a competitive enzyme immunoassay. Competition occurs between the antigen (present in standards, controls and patient samples) and an enzyme-labeled antigen (conjugate) for a limited number of antibody binding sites on the microplate. After samples and conjugate have been incubated for one hour, washing of the microplate removes unbound materials and an enzyme substrate that generates color is added. The enzymatic reaction is terminated by addition of stopping solution. The optical density, measured with a microplate reader, is inversely proportional to the concentration of 17α-Hydroxyprogesterone (17α-OHP) in the sample. A set of standards is used to plot a standard curve from from which the concentration of 17α-Hydroxyprogesterone (17α-OHP) in samples and controls can be directly read.
Specimen Collection and Storage
Approximately 0.05 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labeled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower temperature for longer time. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.