17α-Hydroxyprogesterone ELISA Assay Kit

$190.00

The Eagle Biosciences 17α-Hydroxyprogesterone (17α-OHP) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of 17α-Hydroxyprogesterone in human serum. The 17α-Hydroxyprogesterone (17α-OHP) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: OHP31-K01 Categories: , ,

17α-Hydroxyprogesterone ELISA Assay Kit

The 17α-Hydroxyprogesterone ELISA Assay Kit is for Research Use Only

Size: 12 x 8 wells
Sensitivity: 0.058 ng/mL
Dynamic Range: 0.05–30 ng/mL
Incubation Time: 1 hour 20 minutes
Sample Type: Serum
Sample Size: 20 µL
Controls Included


Assay Principe for 17α-Hydroxyprogesterone ELISA

The Eagle Biosciences 17α-Hydroxyprogesterone (17α-OHP) ELISA is a competitive enzyme immunoassay. Competition occurs between the antigen (present in standards, controls and patient samples) and an enzyme-labeled antigen (conjugate) for a limited number of antibody binding sites on the microplate. After samples and conjugate have been incubated for one hour, washing of the microplate removes unbound materials and an enzyme substrate that generates color is added. The enzymatic reaction is terminated by addition of stopping solution. The optical density, measured with a microplate reader, is inversely proportional to the concentration of 17α-Hydroxyprogesterone (17α-OHP) in the sample. A set of standards is used to plot a standard curve from from which the concentration of 17α-Hydroxyprogesterone (17α-OHP) in samples and controls can be directly read.


Specimen Collection and Storage

Approximately 0.05 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labeled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower temperature for longer time. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.


Related Products

17-OH Progesterone ELISA Assay Kit
Progesterone ELISA Assay Kit

Additional Information

Assay Background


The steroid 17α-Hydroxyprogesterone is produced by the adrenal cortex and gonads. 17α-OHP has little progestational activity, but has intense clinical interest because it is the immediate precursor to 11-desoxycortisol, which is produced by the 21-hydroxylation of 17α-OHP. Measurement 17α-OHP is, consequently, a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17α-OHP is secreted in abundant excess. Measurement of 17α-OHP is therefore valuable in the initial diagnosis of CAH.

Typical Standard Curve


Documents

Product Manual