Free Estriol Serum ELISA Assay Kit
The Free Estriol Serum ELISA Assay Kit is for Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.058 ng/mL
Dynamic Range: 0.05–30 ng/mL
Incubation Time: 1 hour 20 minutes
Sample Type: Serum
Sample Size: 20 µL
Assay Principe for Free Estriol Serum ELISA
The Free Estriol (also referred to as unconjugated estriol or uE3 in the literature) ELISA is a competitive immunoassay. Competition occurs between Estriol present in calibrators, controls, and samples and an enzyme-labeled antigen (conjugate) for a limiting number of anti-Estriol antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added, and approximately 15-20 minutes late the enzymatic reaction is terminated by addition of stopping solution .The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of Estriol in the sample. A calibrator curve is plotted with a provided set of calibrators to calculate directly the concentration of estriol in samples and controls.
Specimen Collection and Storage
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labeled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done later. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.