Eagle Bio Antibodies

Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY)

$610.00

The Eagle Biosciences Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY) is for research use only.

SKU: 305-100 Categories: ,

Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY)

For Research Use Only

Immunogen:   Human GFRa-3

Uniprot ID:    O60609

Alternative Names:    GDNF receptor alpha-3

Clonality:    Chicken polyclonal

Class:   IgY

Reactivity:   Human GFRa-3,doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4

Application:  ELISA, WB, IF

Purification:   GFRα-3 affinity purified

Buffer:    Concentrated ammonium sulphate in PBS pH 7.4

Concentration:    1 mg/ml

Unit Size:   100 µg

Related Products:    Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors.

Shipping:    This product is shipped in non-frozen liquid form in ambient conditions.

Storage:   Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Immunogen Description:

Recombinant His-tagged human GFRa-3 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Protocol:

Western immunoblotting, recommended dilution 1:5 000 to 1:10 000; ELISA: 1:160 000 to 1:320 000, IF 1:1500 to 1:3000. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background:  

The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Additional Information

Protocol:Western immunoblotting, recommended dilution 1:5 000 to 1:10 000; ELISA: 1:160 000 to 1:320 000, IF 1:1500 to 1:3000. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background:
The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Figure 1:

IF detection of human GFRα-3 expressed in U2OS cells. GFRα-3 was visualized using Chicken polyclonal antibody to human GFRα-3 (dilution 1:3000) and Goat anti-chicken IgY – H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-3 specific signal and blue for nuclei.

Figure 2:

Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-3. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen)  was used as secondary antibody.

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