Total GIP NL-ELISA Assay Kit
The Total GIP NL-ELISA Assay Kit was manufactured in Sweden by Mercodia
Size: 12 x 8 wells
Sensitivity: 1.62 pmol/L
Dynamic Range: 2.7 – 1000 pmol/L
Incubation Time: Overnight
Sample Type: Serum, EDTA plasma
Sample Size: 25 µL
Assay Background
Glucose dependent insulinotropic polypeptide (GIP) is an incretin, a gut-derived circulating peptide hormone that potentiates glucose-dependent insulin secretion following ingestion of a meal1. GIP(1-42) (active GIP) is rapidly cleaved in circulation at the N-terminus by DPP-4 to yield GIP(3-42), regarded as a non-active form of GIP2. Because of this quick proteolytic degradation, not only intact but also total (i.e. intact plus DPP-4-metabolized) forms of GIP must be measured to study its secretion and processing in vivo.
Assay Principle
The Total GIP NL-ELISA Assay Kit is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the GIP molecule. GIP in the sample reacts with anti-GIP antibodies bound to microtitration wells and peroxidase-conjugated anti-GIP antibodies in the solution. A simple washing step removes unbound enzyme-labelled antibody. The bound conjugate is detected by reaction with the chemiluminescent substrate. A luminescence plate reader is used to read the intensity of light generated.
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