Glucagon Control Set

$120.00

The Glucagon Control Set can be used with the Glucagon ELISA Assay Kit (10-1271-01) and the Mouse/Rat Glucagon ELISA Assay Kit (10-1281-01).

SKU: 10-1286-01 Categories: , ,

Glucagon Control Set

The Glucagon Control Set is For Research Use Only

SIZE: 3 vials, 0.5 mL after reconstitution


Assay Background

Glucagon is a 29 amino acid polypeptide processed from proglucagon (residues 33-61) in pancreatic alpha cells. In intestinal L-cells proglucagon is cleaved into glicentin, corresponding to proglucagon residues no. 1-69. Glicentin can further be processed into oxyntomodulin, corresponding to proglucagon residues no. 33-69. Moreover, a fragment of glucagon corresponding to its C-terminal part (residues no. 19-29), also designated mini-glucagon, is reported to be present in the pancreas in low amounts compared to the total glucagon content. In general, glucagon has an effect opposite that of insulin, i.e. it raises blood glucose levels. It causes the liver to convert glycogen into glucose, which is then released into the blood stream. With longer stimulation, glucagon action in the liver results in a glucose-sparing activation of free fatty acid oxidation and production of ketones. During hypoglycaemia, glucagon secretion offers a protective feedback mechanism, defending the organism against damaging effects of glucose deficiency in the brain and nerves.


Preparation of reagents

Reconstitute each Glucagon Control (Low, Medium, High) by adding 500 µL redistilled water to each vial. Replace the rubber stopper and let stand for 5 minutes. Gently swirl until contents are thoroughly mixed. Avoid prolonged exposure to room temperature.

Shelf life and storage

The expiry date is stated below. Store the complete package at 2–8°C. Reconstituted controls are stable for one week at 2–8°C. If reconstituted controls are to be used for longer than one week, aliquot and store at -20°C. Aliquoted controls are stable for 4 weeks at -20°C. Avoid repeated Freeze/Thaw cycles.


Related Products

Glucagon ELISA Assay Kit
Mouse/Rat ELISA Assay Kit

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations