Human MIA ELISA Kit
The Human MIA ELISA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
Sample Size: 100 µl
Alternative Names: Melanoma Inhibiting Activity, Cartilage-derived Retinoic Acid-sensitive Protein, CD-RAP
Assay Principle
The Human MIA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MIA with a 96-well strip plate that is pre-coated with antibody specific for MIA. The detection antibody is a biotinylated antibody specific for MIA. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MIA with immunogen: Expression system for standard: E.coli; Immunogen sequence: G25-131Q. The kit is analytically validated with ready to use reagents. To measure Human MIA, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MIA in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MIA in the sample.
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