Human MCAM ELISA Kit

$515.00

The Human MCAM/CD146 ELISA Kit is intended for the quantitation of Human MCAM concentrations in cell culture supernates and serum. The Eagle Biosciences Human MCAM/CD146 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: MCM31-K01 Categories: , ,

Human MCAM ELISA Kit

The Human MCAM ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and serum
Sample Size: 100 µl
Alternative Name: CD146, Cluster of Differentiation 146, Melanoma Cell Adhesion Molecule, Cell Surface Glycoprotein MUC18


Assay Principle

The Human MCAM Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MCAM with a 96-well strip plate that is pre-coated with antibody specific for MCAM. The detection antibody is a biotinylated antibody specific for MCAM. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human MCAM with immunogen: Expression system for standard: NSO, Immunogen sequence: V24-G559. The kit is analytically validated with ready to use reagents. To measure Human MCAM, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MCAM in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MCAM in the sample.


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Additional Information

Assay Background


CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. By radiation hybrid analysis, it mapped the CD146 gene to chromosome 11q23.3. It is a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.

Typical Standard Curve


Human MCAM ELISA Kit

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