Urine Creatinine Detection Kit

Additional Information

Assay Principle

A creatinine standard, calibrated to a NIST creatinine stan-dard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressX-press® Creatinine Reagent, which is pipetted into each well. After a short incuba-tion the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine7,8.

Typical Standard Curve

Assay Background

Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phospho-creatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist2. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.

Sample Types
Sample Types Validated:
Human, Monkey, Dog, and Rat Urine
This assay has been validated for human, rat, dog and monkey urine samples. Urine samples containing visible protein or particulates should be centrifuged or filtered prior to using. Mouse urine samples are not compatible with the use of this assay to determine GFR as over half of murine urinary creatinine is from renal secretion rather than filtration.