The Vitamin Assay Kit line offered Eagle Biosciences provides unique and sensitive assays for measuring a variety of vitamin analytes. Our Biotin ELISA Assay Kit is a top-notch assay that provides a method that is easy to use, cost effective, and provides quantifiable results in under 3 hours. This kit has proven to be a valuable tool to researchers in various fields of study.
There is one recent study described below utilizing our Biotin ELISA Assay Kit, designed for the quantitative determination of Biotin (Vitamin H, Coenzyme R, Vitamin B7) in food products, cell culture, and tissue samples.
Publication:
Study Summary:
In normal adult cells, the structure at the ends of chromosomes, called the telomere, becomes progressively shorter with each replication cycle. When telomeres get too short or damaged, the cell stops dividing and becomes senescent. The enzyme telomerase maintains the integrity of telomeres, and phosphorylation stabilizes the catalytic subunit (called TERT) of this enzyme. In both normal fibroblasts and lung cancer cells, Panneer Selvam et al. found that binding of the phospholipid S1P to TERT mimicked protein phosphorylation and prevented TERT degration. Disrupting the interaction between S1P and TERT by either depleting S1P or mutating the interaction site in TERT impaired telomere maintenance and promoted senescence in cultured cells and decreased the growth of lung cancer cell xenografts in mice. Researchers utilized Eagle Biosciences’Biotin ELISA Assay Kit to measure levels of Biotin in Biotinylated S1P–bound hTERT proteins.
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