ELISA Archives

Comparison between the AroCell TK 210 ELISA and TK1 enzyme activity assays

AroCell recently completed a comparison between their 210 ELISA with the ABCAM TK1 ELISA and the Gold-Standard Tritiated TK1 Activity Assay to confirm the diagnostic accuracy of each kit. The results found that the AroCell kit is an accurate assay for the determination of thymidine kinase 1.

Read the report here.

Eagle Biosciences offers the AroCell TK 210 ELISA as part of our product catalog:
TK 210 (Thymidine Kinase 1 (TK1)) ELISA Assay Kit

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Eagle Welcomes Fianostics!

by Eagle Biosciences

Eagle Biosciences, Inc. is excited to collaborate with Austrian biotech Fianostics, to offer a new fluorescence-based detection platform for immunoassays, called FluoBolt™, which enables highly sensitive assays with high reproducibility and reliability. This dramatically improves the informative value of research with biomarkers.

So far, Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays have been developed to detect

Asporin
Noggin
Periostin
and many more to come!

What Makes These Assays Different?

Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays have been established to eliminate the complicated processes that come with your standard sandwich ELISA. By using their FluoBolt™ Metal Enhanced Fluorescence Immunoassays, you can expect

High Sensitivity!
Single Step Assay Procedure!
No Enzyme Substrate!
Stable Signal over Time!
100% Compatibility with 96-well ELISA Format!

About Fianostics

Fianostics combines scientific and technical expertise in diagnostics with the development of a high-tech detection platform that benefits in reproducibility from the excellent expertise of Sony DADC BioSciences in the production of polymer consumables for the diagnostic industry. Their know-how is not limited to the development of the new detection platform, but we also provide the diagnostic application with the appropriate immunoassays for specific clinical areas. (for research use only in the US)

About Metal Enhanced Fluorescence

Metal Enhanced Fluorescence (MEF) offers the possibility to dramatically increase the analytical sensitivity of systems based on fluorescence detection. MEF is based on the fact that excitation light interacts with the electrons of metal nano–structures thus generating very high electromagnetic fields (Localised Surface Plasmons, LSPs ) Therefore, such structures are also called

”plasmonic structures” and the combination of (e.g, polymeric) support and structure is known as “plasmonic substrate”. These LSPs lead to an increase in emission output of fluorescent molecules (e.g. fluorescently labelled antibodies) when bound to surfaces with suitable nano-metal structures that can enhance the signal more than 100 times.

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We Have A New Look!

by Eagle Biosciences

Hello Loyal Customers!

Our team here at Eagle Biosciences, Inc. are so excited to introduce our new look for you to enjoy! The new EagleBio.com comes chocked-full of new features that will make your browsing and shopping experience far superior to what you’re used to!

Speak with one of our team members online when you need help
Fast, easy and secure check out
Smooth navigation while browsing one of our many unique products

But That’s Not All…

Not only have we made your shopping experience better when you choose to order from Eagle Biosciences, but we are also working with some phenomenal new partners to bring you new and unique products! Here’s a sneak peak…

Fianostics FluoBolt™-FIAs : Metal enhanced fluorescence assays that offers a higher sensitivity, single-step alternative to your basic ELISA. Available for detection of Noggin, Asporin, and Periostin! As well as services to test a variety of other markers!
osteomiR testing by TAmiRNA : Workflow that detects 11 microRNA associated with bone quality. We will feature osteomiR Test Kit, as well as services that can run your samples for you.
New and unique Drug Monitoring Assays

As always, we appreciate your business! Without you we wouldn’t be able to do what we love by providing you the products you need to change the future! Be sure to keep an eye out for more to come.

If you want to see anything from Eagle Biosciences (products, services, etc.) Let us know! We’re here for you!

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UPDATE: Calretinin ELISA Assay Used in a New Study!

by Eagle Biosciences

EagleBio’s Calretinin ELISA Assay Kit was recently used in a study for the detection of malignant mesothelioma. This assay is a part of our Cancer Biomarker Kit line which is comprised of unique,versatile set of products for a variety of applications, research, and fields of study. Check out the product pages for full details on the kit or view the links below to find a new publication referencing our Calretinin ELISA Assay Kit.

Johnen, G., Gawrych, K., Raiko, I,. et al. “Calretinin as a blood-based biomarker for mesothelioma.” BMC Cancer. (2017) Vol. 17:386

“Concentrations of calretinin in plasma and serum samples were determined as described. In brief, a 1:1500 dilution of purified rabbit polyclonal anti-calretinin was used as capture antibody and a 1:5000 dilution of biotinylated polyclonal anti-calretinin as detection antibody. Samples (plasma or serum) were diluted 1:5 in Tris-buffered saline supplemented with 5 mM CaCl2. A volume of 100 μl of a diluted sample was used for each determination. Calretinin concentrations were determined from a standard curve of human purified recombinant calretinin diluted between 10 and 0.08 ng/mL run in parallel on each plate. All determinations of calretinin were performed in the laboratory of the IPA.” Read More.

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Maryland Study: EagleBio’s IGF-1 BIOTINYLATED (BIOTIN IGF-1)

by Eagle Biosciences

IGF-1 BIOTINYLATED (BIOTIN IGF-1) is for Research Use Only. Not for diagnostic procedures.

Eagle Biosciences’ IGF-1 BIOTINYLATED (BIOTIN IGF-1 was recently utilized in a study conducted at Frederick National Laboratory for Cancer Research in Maryland. Our Biotin- R3 IGF-I is prepared by a selective biotinylation procedure and is offered in a stable and convenient lyophilized solution. Our technology permits biotinylation without loss of binding capacity to
IGFBP´s or IGF antibodies. Potential Applications: Western-ligand blotting,Western-ligand blots with biotinylated IGF-I and analogs, and ELISAs.

Publication:

Mammalian cell transient expression, non-affinity purification, and characterization of human recombinant IGFBP7, an IGF-1 targeting therapeutic protein

It has been well documented that insulin-like growth factor 1 (IGF-
1) plays multiple roles during cancer development. Therefore, targeted
manipulation of IGF-1 signal pathway is an attractive strategy for cancer
treatment. Insulin-like growth factor-binding protein 7 (IGFBP7), a nonIgG
protein, interacts directly with IGF-1 and VPS24. Read More

(Source:https://www.researchgate.net/publication/282944657)

Related kits:

MULTISPECIES IGF-1 ELISA

Related News:

Cancer treatment on a cellular level

October 1, 2012: Eagle Biosciences Introduces Recombinant Human Insulin, Biotin-labeled (Biotinylated Insulin)