Xanthurenic Acid Monoclonal Antibody
The Xanthurenic Acid Monoclonal Antibody is For Research Use Only
The Eagle Biosciences first and only validated anti-Xanthurenic acid antibody available for research use. Internal validation for IHC and IF of the 6C12-A12 monoclonal antibody highlighted cytoplasmic presence of xanthurenic acid in human midbrain tissues.
Synonyms: Anti-Xanthurenic acid antibody, 8-Hydroxy-4-oxo-1H-quinoline-2-carboxylic acid antibody, Xanthuric acid antibody, Xanthurenate antibody, 8-Hydroxykynurenic acid antibody
Immunogen: Conjugated Xanthurenic acid
Clone: Clone 6C12-A12
Specificity: When tested in competitive ELISA, the anti-conjugated Xanthurenic acid antibody did not show any significant cross-reactivity with competitors Kynurenic acid and Quinaldic acid conjugates.
Size: 0.5 mg/mL
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water.Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
Affinity & Specificity of Anti-Xanthurenic Acid Antibody
Competitive ELISA demonstrates that moderate amount of cinnabarinic acid conjugate is required to abolish antigen-antibody reaction (satisfying affinity), while rising concentrations of 3-OH-Anthranilic acid conjugate do not affect the reaction (high specificity).
Immunohistochemistry (IHC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate