Cinnabarinic Acid Monoclonal Antibody
The Cinnabarinic Acid Monoclonal Antibody is For Research Use Only
The Eagle Biosciences first and only validated anti-Cinnabarinic acid antibody available for research use. IHC validation of the 5C5-E10 mAb in human brain and breast tumor tissues revealed the presence, in specific cells, of Cinnabarinic acid, a tryptophan metabolite known for its immunomodulatory role.
Synonyms: 2-amino-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid antibody
Immunogen: Conjugated Cinnabarinic acid
Isotype: IgG1 k chain
Clone: Clone 5C5-E10
Specificity: When tested in competitive ELISA, the anti-Cinnabarinic acid antibody 5C5-E10 did not cross-react with its precursor, 3-OH-Anthranilic acid conjugates
Size: 0.5 mg/mL
Storage: 4°C
Specificity of Anti-Cinnabarinic Acid Antibody
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
Competitive ELISA demonstrates that moderate amount of cinnabarinic acid conjugate is required to abolish antigen-antibody reaction (satisfying affinity), while rising concentrations of 3-OH-Anthranilic acid conjugate do not affect the reaction (high specificity).
Protocols
Immunohistochemistry (IHC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
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