Wide Range Rat Insulin ELISA Assay Kit


The Wide Range Rat Insulin ELISA Assay Kit is designed for quantification of mouse insulin in serum, plasma and cell culture samples. The Eagle Biosciences Wide Range Mouse Insulin ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

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Wide Range Rat Insulin Assay ELISA Kit

The Wide Range Rat Insulin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 1ng/mL
Standard Range: 1ng/mL-50ng/mL
Incubation Time: 1 hours 45 minutes
Sample Type: Serum, plasma, and fluid
Sample Size: 5 µl

Assay Principle

The Wide Range Rat Insulin assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Sample Handling

No dilution of the sample is required in this assay, if a sample has an insulin
level greater than the highest standard, the sample should be diluted with 0
ng/ml insulin standard solution and the assay should be repeated.

Related Products

Insulin Ultrasensitive ELISA Assay Kit
Human Insulin Biotinylated (Biotin-Insulin)
IAA (Insulin Autoantibodies) ELISA Assay Kit

Additional Information

Typical Standard Curve

Wide Range Rat Insulin ELISA Kit


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