Vitamin B2 HPLC Column


The Vitamin B2 HPLC Column can be used with Eagle Biosciences Vitamin B2 HPLC Assay (VB231-H100). Each column can have approximately 500-1000 samples.

SKU: IC2300rp Categories: , , Tag:

Vitamin B2 HPLC Column

The Vitamin B2 HPLC Column is For Research Use Only

Each column is can be utilized for approximately 500-1000 samples depending on column care and sample type.

Assay Background

Vitamin B2 is a collective term for the molecules Riboflavin, Flavin Adenine Dinucleotide (FAD) and Flavin Adenine Mononucleotide (FMN). Riboflavin belongs to the group of Flavonoids (plant pigments). It only can be synthesized by plants and microorganisms. Humans and animals ingest it via the gut and convert it to the coenzymes FAD and FMN. They are distributed within the whole body with maximum concentrations in liver, kidney and heart.

FAD and FMN are important coenzymes in protein and energy metabolism. In the central nervous system vitamin B2 is involved in the control of neurohormones and biogenic amines.  A lack of vitamin B2 leads to unspecific symptoms as fatigue and listlessness. Also, erythema, itching and gingival inflammation are recognized. The metabolic pathways of carbohydrates, fatty acids, proteins, and iron and vitamin B6 metabolism are affected. A serious lack of the B2 vitamins leads to inflammation of the mucous membrane in mouth and nose, skin disease (dermatitis), ophthalmic disorder (reduction of the acuteness of vision, cataract), neurological disorders and disturbance of the iron metabolism.

Vitamin B2 (Riboflavin) HPLC Assay kit makes it possible to determine the vitamin in an easy, fast and precise way. Vitamin B2 (Riboflavin) HPLC Assay kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the columns (IC2300rp) and the controls (IC2300ko). Both can be supplied by Eagle Biosciences.  Beside the complete test kits it is possible to order all components separately. Please request our single component price list.

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