Vitamin B2 HPLC Assay
The Vitamin B2 HPLC Assay is For Research Use Only
Size: 100 tests
Sensitivity: 1.0 pg/mL
Incubation Time: 5 minutes
Sample Type: EDTA Whole Blood
Sample Size: 200 µL
Alternative Name: Riboflavin
Product Developed and Manufactured in Germany
Vitamin B2 is a collective term for the molecules Riboflavin, Flavin Adenine Dinucleotide (FAD) and Flavin Adenine Mononucleotide (FMN). Riboflavin belongs to the group of Flavonoids (plant pigments). It only can be synthesized by plants and microorganisms. Humans and animals ingest it via the gut and convert it to the coenzymes FAD and FMN. They are distributed within the whole body with maximum concentrations in liver, kidney and heart.
FAD and FMN are important coenzymes in protein and energy metabolism. In the central nervous system vitamin B2 is involved in the control of neurohormones and biogenic amines. A lack of vitamin B2 leads to unspecific symptoms as fatigue and listlessness. Also, erythema, itching and gingival inflammation are recognized. The metabolic pathways of carbohydrates, fatty acids, proteins, and iron and vitamin B6 metabolism are affected. A serious lack of the B2 vitamins leads to inflammation of the mucous membrane in mouth and nose, skin disease (dermatitis), ophthalmic disorder (reduction of the acuteness of vision, cataract), neurological disorders and disturbance of the iron metabolism.
Vitamin B2 (Riboflavin) HPLC Assay kit makes it possible to determine the vitamin in an easy, fast and precise way. Vitamin B2 (Riboflavin) HPLC Assay kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the columns (IC2300rp) and the controls (IC2300ko). Both can be supplied by Eagle Biosciences. Beside the complete test kits it is possible to order all components separately. Please request our single component price list.