Vasoactive Intestinal Peptide (VIP) ELISA Assay Kit
The Vasoactive Intestinal Peptide (VIP) ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Standard Range: 0 – 800 pg/mL
Incubation Time: 2 hours 30 minutes
Sample Type: EDTA Plasma
Sample Size: 75 µL
Controls Included
Assay Principle
The VIP ELISA is a two-step competitive immunoassay. In the first incubation step, competition occurs between VIP present in calibrators, controls, specimen samples and a biotin-labeled antigen (biotin conjugate) for a limited number of anti-VIP antibody binding sites on the microplate wells. Excess and unbound materials are removed by a washing step. In the second incubation step, streptavidin-HRP conjugate is added, which binds specifically to any bound biotin conjugate. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of VIP present. Following an incubation, the enzymatic reaction is terminated by the additional of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of VIP in specimen samples and controls can be directly read.
