Human TRAIL R1 ELISA Assay


The Eagle Biosciences Human TRAIL R1/DR4 ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of TRAIL R1/DR4 in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human TRAIL R1/DR4. The Eagle Biosciences Human TRAIL R1/DR4 ELISA Assay kit is for Research Use Only.

SKU: C6131-K01 Categories: , ,

Human TRAIL R1 ELISA Assay

The Human TRAIL R1 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 8 ng/ml
Dynamic Range: 31.25 – 1000 ng/ml
Incubation Time: 2h 30 min
Sample Type: Serum, Cell Lysates, Cell Culture
Sample Size: 100 µl
Alternative Names: CD261, DR4, Death Receptor 4, APO2 ligand Receptor 1, Tumor necrosis factor-related apoptosis-inducing ligand receptor 1

Assay Background

Human TRAIL R1/DR4, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for TRAIL (APO2 ligand). TRAIL R1 is involved in apoptosis. In the TRAIL receptor family, TRAIL R1, but also TRAIL R2 (DR5) transduce an apoptosis signal, whereas TRAIL R3 (DcR1) and TRAIL R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. TRAIL R1 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. TRAIL R1 shares 55% of its amino acid sequence with TRAIL R2. TRAIL R1 is widely expressed in normal tissue.

Storage Instructions
Store the Human TRAIL/R1/DR4 ELISA Assay kit reagents between 2 and 8°C. Immediately after use remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the Human TRAIL/R1/DR4 ELISA Assay kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.

Related Products

Human TRAIL / R2/DR5 ELISA Assay Kit
Human TRAIL / R3/DcR1 ELISA Assay Kit
Human TRAIL/R4/DcR2 ELISA Assay Kit

Additional Information

Assay Principle

A capture Antibody highly specific for the Eagle Biosciences TRAIL R1 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of TRAIL R1 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti- TRAIL R1 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of TRAIL R1 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of TRAIL R1 in any sample tested.

  1. Add 100µl of standard diluent to zero wells and 100µl of Samples to sample wells
  2. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hour(s)
  3. Remove the cover and wash the plate
  4. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hour(s)
  5. Repeat wash step 3.
  6. Add 100μl of Streptavidin-HRP solution into all wells
  7. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  8. Repeat wash step 3.
  9. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  10. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil.
  11. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve

Package Inserts

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