cAMP Plasma ELISA Assay Kit
The cAMP Plasma ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Standard Range: 1 – 100 pmol/mL
Incubation Time: 1 hour 20 minutes
Sample Type: Plasma
Sample Size: 100 µL
Controls Included
Assay Principle
The cAMP Plasma ELISA is a competitive immunoassay. Competition occurs between cAMP present in calibrators, controls, specimen samples and an enzyme-labeled antigen (HRP conjugate) for a limited number of anti-cAMP antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of cAMP present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of cAMP in specimen samples and controls can be directly read.
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