Anti-ASGPR ELISA Assay Kit
Anti-ASGPR ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: Cut-off Controls
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Names: Asialoglycoprotein Receptor ELISA, Human Anti-ASGPR ELISA
For Research Use Only
The high quality of the immobilized ASGPR ensures the exclusive reaction of ASGPR autoantibodies.
Negative: < 0.9
Positive: ≥ 1.1
Grey Zone: 0.9 – 1.1
It is recommended that each laboratory establishes its own normal and pathological reference ranges for serum Anti-ASGPR (Asialoglycoprotein Receptor) ELISA Assay Kit levels. Therefore, the above mentioned reference values provide a guide only to values which might be expected.
This Anti-ASGPR ELISA Assay Kit (Asialoglycoprotein Receptor) is an enzyme immunoassay for the semi-quantitative determination of IgG antibodies to ASGPR. The antibodies of the calibrators and diluted samples react with ASGPR immobilized on the solid phase of microtiter plates. ASGPR highly purified from rabbit liver and coated on the microtiter plate guarantees the specific binding of ASGPR IgG antibodies of the specimen under investigation. Following an incubation period of 60 min at 37 °C, unbound serum components are removed by a washing step.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within an incubation period of 30 min at 37 °C. Excessive conjugate is separated from the solid-phase immune complexes by the following washing step. Horseradish peroxidase converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound.