Thymulin ELISA Assay Kit
Thymulin ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Standard Range: 0.03-16 ng/ml
Incubation Time: 1h; 16h; 1h; 15min
Sample Type: Serum, Thymus extract
Sample Size: 50 µL
For Research Use Only
Controls Included
h3>Assay Principle
The Thymulin ELISA is designed for the quantitative determination of Thymulin in serum and thymus preparations. The test principle is based on a competition between the antigen in the sample or standards and biotinylated thymulin as a tracer for the binding sites of anti-Thymulin antibodies coated on the wells of the microplate. A peroxidase-conjugated steptavidin is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. Thymulin present in the patient samples is determined directly from this curve.
Possible research areas:
• Immune dysfunction and immune deficiencies, e.g. leukaemia, AIDS
• Autoimmune disease, e.g. systemic Lupus erythematodes, rheumatoid arthritis, multiple sclerosis
• Zinc dependent diseases, e.g. Morbus Crohn
• Dysfunction of the endocrinium
• Quality control of thymus preparations
Thymulin ELISA Test Quality Control
The use of external controls for the Thymulin ELISA is recommended for internal quality control, if possible. Control samples should be analysed with each run. Results, generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the patient samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
Reference range
Baseline values depend on the patient’s age and vary between different individuals. We recommend each laboratory to establish its own reference range.
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